The NDP supported the banning of conversion therapy and enshrinement of transgender human rights protections. Both of these policy issues were initially introduced as Private Members Bills by NDP MPs. The NDP has consistently urged greater support for LGBTQ refugees, as well as greater funding for 2SLGBTQIA civil society organizations, nonprofits and charities. Throughout their history, the NDP have often been the first party on the provincial and federal level to explicitly support 2SLGBTQIA+ human rights and policies that advance equality for 2SLGBTQIA+ people.
Many NDP MPs, including the leader, vocally opposed policies related to transgender people, youth and families introduced in New Brunswick, Saskatchewn and Alberta. They have also criticized policies related to transgender and queer issues introduced by American President Donald Trump. The NDP were the first party with an openly queer MP and the first with an openly Two-Spirit MP.
The NDP has pledged to improve access to gender-affirming healthcare, with a statement reading that “make sure people can get the gender-affirming care they need, and work with trans and gender-diverse organizations and fight to combat gender-based and trans-based violence”.
The NDP platform for the 2025 federal election made few specific commitments on 2SLGBTQIA+ issues, but it did include a line stating that they "would take new steps to protect diversity in Canada, including supporting 2SLGBTQI+ communities who are increasingly subjected to hate and to violence"